Humans enact the function of PTP-MEG2 in secretory-vesicle fusion with the plasma membrane

Delight and Edmore

Delight and Edmore as PtdIns 3 & 4

 

 

Mr Garikai in full PPE

Mr Garikai in full PPE as PtdIns 5

 

Out of the heat haze

Out of the heat haze

 

Inactive NSF (N-ethylmaleimide-sensitive factor)

Inactive NSF (N-ethylmaleimide-sensitive factor)

 

Inactive NSF (N-ethylmaleimide-sensitive factor)

Inactive NSF (N-ethylmaleimide-sensitive factor)

 

 

Take your positions directs Mr Ramius

 

 

Stone built plasma membrane

Stone built plasma membrane

DSCF3421

 

 

 

The formation of a secretory vesicle

The formation of a secretory vesicle

 

And, finally, the obligatory puppy pic (for Sue)

Hamlet and Brutus

Hamlet and Brutus

 

See WengLong, Joachim, and Hilary – I must have been paying attention at some time!

 

 

 

Rondavel extension part 4: Foundation Walls

Although these look like walls – they are in fact the foundation walls build on top of the footings  i.e. they will be below the level of the final building ground floor.

 

Here is the idea in progress – the foundation walls now part way up.

 

Rondavel extension foundation walls in progress

Rondavel extension foundation walls in progress

 

and here, showing a temporary retaining wall in stone immediately behind the builders

Rondavel extension foundation walls in progress

Rondavel extension foundation walls in progress

 

Next some details of the building process when working in stone as we are here.

 

Farai begins his wall

Farai begins his wall

 

Of course the stones don’t come in  pre-packed sizes…so yes they have to be first burnt, then hammered and finally, artfully positioned so that they fit and the ‘look’ from the outside or inside is ‘good’

Hammering stone to size

Hammering stone to size

 

 

Mr Patrick Samhembere in action

Mr Patrick Samhembere in action

Mr Patrick Samhembere in action

Mr Patrick Samhembere in action

 

Mr Patrick Samhembere in action

Mr Patrick Samhembere in action

The hands that built Hornbydale

The hands that built Hornbydale

 

The hands that built Hornbydale

The hands that built Hornbydale

 

 

After a day the spare cement is scraped away

After a day the spare cement is scraped away

 

Mr Perkins Makore

Mr Perkins Makore – builders assistant

 

Mr Farai Samhembere - builder

Mr Farai Samhembere – builder

 

Mr Farai Samhembere - builder

Mr Farai Samhembere – builder

Mr Mabika - builder

Mr Mabika – builder

 

Mr Tendai Mushonga - builders assistant

Mr Tendai Mushonga – builders assistant

 

Mr Tendai Samhembere - builder

Mr Tendai Samhembere – builder

 

Mr Tendai Samhembere - builder

Mr Tendai Samhembere – builder

 

Nearly there on the foundation wall

Nearly there on the foundation wall

Finished section of foundation wall

Finished section of foundation wall

 

 

Tendai enjoying having his picture taken!

Tendai enjoying having his picture taken!

 

Update:

 

The completed  ‘slab’ on top of the foundation walls

Completed slab on top of foundation walls

Completed slab on top of foundation walls – looking west

 

Completed slab on top of foundation walls

Completed slab on top of foundation walls – looking east

Tadpole Part 7: The Before & The After

Here are some ‘before’ and ‘after’ shots – so we can see how far we’ve come since 1st April 2014!

 

At the beginning –  a burnt out shell of a building

A burnt out roofless structure

A burnt out roofless structure

 

and suddenly ..

The tadpole building

The tadpole building

 

 

Walls go up and extend the original rondavel

Walls go up and extend the original rondavel

Looks better now

Looks better now

 

looking south

looking south

 

at sunset now

at sunset now

 

the original doorway

the original doorway

and now its an interior doorway!

and now its an interior doorway!

And again from the inside – the original door and window to its left.

that original doorway from the inside

that original doorway from the inside

 

our masonry stove - faced in soap stone

our masonry stove – faced in soap stone

Remember the saga of the stairs:

Mr Willard infront of the eventual stairs

Mr Willard infront of the eventual stairs

 

Well we got there in the end!

stairs - unfinished

stairs – unfinished

Finished stairs

Finished stairs

 

Looking down those stairs

Looking down those stairs

And finally the school room up those stairs

 

Montessori school room upstairs in Tadpole

Our Montessori school room upstairs in Tadpole

 

The Scottish play in our school library

The Scottish play in our school library

 

Rondavel extension Part 2: The foundation trenches

After burning some of the granite rock out of the way we start digging. How hard can it be after all?

Mr Tongai & Mr Didymus in the easy trench

Mr Didymus & Mr Tongai in the easy trench

 

Edmore getting that earth out

Edmore getting that earth out

and then signing off for the day

and then signing off for the day

Well up here what you see on top is not what is lurking underneath – more rocks.

OK more burning was to come. The rocks are all under ground too

OK more burning was to come. The rocks are all under ground too!

 

after burning - well nearly after

after burning – well nearly after

 

Somethings still alight in there

Somethings still alight in there

And it is a building site with a pretty good view too.

It slowly goes down

It slowly goes down in size

 

We have to dig round out to burn it. Lots of digging

We have to dig round out to burn it. Lots of digging

 

smaller now!

smaller now!

 

Temporary retaining wall

Temporary retaining wall

 

The men who bring the sand and gravel take a look

The men who bring the sand and gravel take a look at progress

 

If this tree was to fall down...

If this tree was to fall down…

 

 

Mr Garikai does the job first

Mr Garikai does the job first

 

and shows us how its done

and shows us how its done

 

He's getting good at this

He’s getting good at this

 

The site is ready

The site is ready

 

Measuring for the footings

Measuring for the footings

Tadpole Part 6: The Stairs

Well the stairs were a bit of a mission in of themselves. To start with they had to follow the curve of the rondavel, plus I had the fine idea to make them wider at the bottom than the top. So what yours truly didn’t realise is that making a single laminated plank a) angle up, then b) curve while angled, and yes c) follow a decreasing arc not a constant curve (the stairs being wider at the bottom) is not straightforward! So the carpenters had to do their best job yet.

Mr Willard infront of the eventual stairs

Mr Willard infront of the eventual stairs

 

Below you can see the laminated plank that was contorted into part of a decreasing 3D spiral

Mr Willard planing

Mr Willard planing

 

Is this really going to work?

Is this really going to work?

 

The Nyakameme brothers do their thing

The Nyakameme brothers do their thing

 

and they make it work!

and they make it work!

 

Mr Willard chiseling

Mr Willard chiseling

 

 

the helical stairs take shape

The helical stairs take shape

 

Bannisters go on

Bannisters go on

 

Edmore at work

Edmore at work

 

Bannisters at dusk

Bannisters at dusk

 

 

Tadpole Part 4: The Roof Goes On

In August 2014 we were high enough to start the roofing activities, build scaffolding, and invent new roofing – cromadek industrial roofing sheet steel cut into a fish scale pattern.

Tadpole dormer window

Tadpole dormer window

 

Constructing the dormer

Constructing the dormer

 

Edmore scales the highest peak

Edmore scales the highest peak while installing Alububble

 

Now Sir Edmore

Now Sir Edmore

 

Covering the dome with Alububble

Lamek helping to cover the dome with Alububble

Lamek on the dome

Lamek on the dome

 

Edmore bringing up the roofing sheets

Edmore bringing up the roofing sheets

Careful handling required

Careful handling required

 

So that it eventually looks like this…….

The Tadpole Building

The Tadpole Building